Andy (sic) blocking could help you with your final deflection numbers. I would consult a local structural engineer and ask for a new blocking layout. Perhaps you may need to double up all the joists.
JW
All blocking does is transfer load to an adjacent joist and keep them from twisting, which maintains whatever vertical strength they have; blocking does NOT decrease the deflection - only shortening or strengthening the joist vertically can do that. Preventing the joists from being able to twist maintains their strength, it does NOT increase it, nor does blocking (except by helping to maintain what strength they have). Keep in mind that the subflooring provides the same general load sharing, and they do not rely on increased strength subflooring to account for joist deflection issues - it is required to provide strength BETWEEN the joists, not along them (especially since the max strength is directional on most subflooring, it is quite a bit weaker along the joist than between them).
Re glue between ply and planks...the whole reason you want the ply there in the first place is to decouple the tile from the seasonal movement of the planks...the planks are likely strong enough in deflection between the joists in the first place. Each stage of decoupling progresses to the maximum stability required for the tile. Gluing the ply to the planks just helps ensure that that movement is transferred through the floor, negating most of the purpose of the ply in the first place. They omit saying to glue on purpose. Same idea with the cbu...testing proves that over time, the screw anchor ends up crushing the cbu since it is tied to the less stable subflooring, but the cbu, if installed properly with the thinset and tape, remains monolithic (albeit with some microscopic crushed holes around the anchors). The thinset underneath cbu is not there to hold it down, it is there to hold it up with 100% support (i.e., vertical deflection). Glue is good between the subflooring and the joists. It is okay between like materials (i.e., ply), but takes careful application with the right materials and technique. All of the TCNA subflooring prep is based on no glue, but, as said, gluing the subfloor to the joists is a good practice - the TCNA generally takes over from a properly installed general application subfloor and converts it to one that can support tile. Laminating ply to ply is a lot of work for not that much improvement (yes, a laminated set of ply IS stronger than those just screwed together), but if you do it wrong, it's worse. And, it makes it a major effort on a remodel down the road as you'd likely have to cut and chop the whole shebang out and start over, increasing costs by a lot. If you had two idential floors, one with blocking and one without and put identical weights in the middle of the span, they would both deflect the same amount assuming that the joists were held properly to the subflooring. The blocking helps to keep them vertical and stable.
Re how to measure for joist deflection - yes, you must measure the joists between structural support members UNDERNEATH the floor (i.e., what is holding it up). Those structures could be the rim joist, a beam, or a structural wall (note, not all walls are built to be structural!). Take a worst cast, a very small room in the middle of the joists. Say the joists can deflect 1/2" under a 'normal' load. Take that same floor and shorten the total length, even though the only area that is tiled is still in the middle, and the deflection may drop to 1/8"...much more tile friendly. Tile is very brittle, nearly any movement will either break the bond, or break the tile, or break the grout, whichever is the weakest. A joist tends to bow - point deflections don't generally happen, so you need to take into consideration the whole length, regardless of where the tile is. Now, if you're only tiling around the perimeter, say right next to the support, you might get away with it since at the supported end it doesn't move much, but that is an atypical situation.